Mycoplasma and Ureoplasma are often  implicated as causative agents in many diseases including vaginosis, nongonococcal urethritis, pylonephritis, pelvic inflammatory disease, postpartum fever and neonatal infections. Polymerase chain reaction (PCR) represents the gold standard for the diagnosis of these pathogens, but is expensive, requires dedicated instrumentations and rooms, skilled technicians and does not provide information about antibiotic sensitivity. Therefore, we developed a laboratory system that allows a fast growth of these microorganisms. With this system Mycoplasma and Ureaplasma grow in wells containing a selective medium and they are visualized by colorimetric reaction. In addition information on antibiotic sensitivity was available. Based on our experience it can be concluded that this assay is useful in a diagnostic setting and suitable for current clinical practice.